Understanding the role of Topoisomerase 2 in chromosome associations
نویسندگان
چکیده
Approved: ____________________________________ Thesis Supervisor ____________________________________ Title and Department ____________________________________ Date 4 UNDERSTANDING THE ROLE OF TOPOISOMERASE 2 IN CHROMOSOME ASSOCIATIONS
منابع مشابه
Xer Recombinase and Genome Integrity in Helicobacter pylori, a Pathogen without Topoisomerase IV
In the model organism E. coli, recombination mediated by the related XerC and XerD recombinases complexed with the FtsK translocase at specialized dif sites, resolves dimeric chromosomes into free monomers to allow efficient chromosome segregation at cell division. Computational genome analysis of Helicobacter pylori, a slow growing gastric pathogen, identified just one chromosomal xer gene (xe...
متن کاملCollision of Trapped Topoisomerase 2 with Transcription and Replication: Generation and Repair of DNA Double-Strand Breaks with 5′ Adducts
Topoisomerase 2 (Top2) is an essential enzyme responsible for manipulating DNA topology during replication, transcription, chromosome organization and chromosome segregation. It acts by nicking both strands of DNA and then passes another DNA molecule through the break. The 5' end of each nick is covalently linked to the tyrosine in the active center of each of the two subunits of Top2 (Top2cc)....
متن کاملDual Role of Topoisomerase II in Centromere Resolution and Aurora B Activity
Chromosome segregation requires sister chromatid resolution. Condensins are essential for this process since they organize an axial structure where topoisomerase II can work. How sister chromatid separation is coordinated with chromosome condensation and decatenation activity remains unknown. We combined four-dimensional (4D) microscopy, RNA interference (RNAi), and biochemical analyses to show...
متن کاملDNA topoisomerase II can drive changes in higher order chromosome architecture without enzymatically modifying DNA.
Topoisomerase II has been suggested to play a major role in chromosome organization based on its DNA decatenating activity and its ability to mediate direct binding interactions between DNA and nuclear matrix. However, this latter point remains controversial. Here we address the question of whether the chromatin binding activity of Topoisomerase II is sufficient to modify chromosome form using ...
متن کاملI-34: NRY Haplotype Analysis: towards A Better Understanding of The Genetic Basis of Spermatogenic Failure
It has been established that the Y chromosome carries genes required for spermatogenesis and male fertility. For many decades worldwide screening for gene identification has been conducted in research laboratories. However, it has been a difficult process in identifying such genes (i.e. causative mutations) which could explain the phenotypic variation and could be potentially used as markers fo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2015